题目: Microbiota of yellow grouper(Epinephelusa woora Temminck&Schlegel,1842)fed two different diets 

作者: Jing-Bin Feng ,Chao-Qun Hu* ,Peng Luo ,Lv-Ping Zhang & Chang Chen 

刊物: Aquaculture Research，（IF=1.186，IV区）

刊号: 2010,41:1778-1790

摘要: The rapidly growing yellow grouper industry has experienced relatively severe bacterial disease problems in China.The proliferation of pathogens in ￠sh can be suppressed by commensal microbiota. In this background, we used nested polymerase chain reactiondenaturing gradient gel electrophoresis (PCR-DGGE) and sequence analysis to investigate microbiota in the skin, gills and intestines, including adherent bacteria and non-adherent bacteria in yellow grouper fed with natural diet and complete feed. A total of 21 bacterial species were identi￠ed using phylogenetic analysis. The g-Proteobacteria group (81.0%, 17 species) dominated the bacterial communities in yellow grouper completely. Others belonged to Firmicutes (9.5%, two species), Actinobacteria (4.75%, one species) and Verrucomicrobia (4.75%, one species). The higher similarities (above 91%) of the DGGE band patterns in skin, gill and intestinal-non-adherent bacteria between two groups of ￠sh indicated that existed more stable microbial communities existed in these speci￠-cally ecological niches in yellow grouper. However, considerable dirences existed between two intestinal-adherent bacteria (IAB) samples; that is, compared with natural diet fed yellow grouper, higher bacterial apparent species richness and possibly less abundance existed in IAB in ￠sh fed with complete diets, probably indicating that the community structures in IAB were acted easily and signi￠cantly by diet.