题目:   Identification of caerulomycin A gene cluster implicates a tailoring amidohydrolase 

作者: Li, J., Long, L.-J., Yang, L.-L., Xu, Y., Wang, F.-Z., Li, Q.-X., Zhang, S*, Li, W.-J* 

刊物: Organic Letters,（IF=5.862，II区）

刊号: 2012, 14(11):2666-2669 

摘要: The biosynthetic gene cluster for caerulomycin A (1) was cloned and characterized from the marine actinomycete Actinoalloteichus cyanogriseus WH1-2216-6, which revealed an unusual hybrid polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) system. The crmL disruption mutant accumulated caerulomycin L (2) with an extended L-leucine at C-7, implicating an amidohydrolase activity for CrmL. The leucine-removing activity was confirmed for crude CrmL enzymes. Heterologous expression of the 1 gene cluster led to 1 production in Streptomyces coelicolor. 

题目:  Spiroindimicins A-D: new bisindole alkaloids from a deep-sea-derived actinomycete 

作者: Wenjun Zhang, Zhong Liu, Sumei Li, Tingting Yang, Qingbo Zhang, Liang Ma, Xinpeng Tian, Haibo Zhang, Caiguo Huang, Si Zhang, Jianhua Ju, Yuemao Shen , and Changsheng Zhang* 

刊物: Organic Letters,（IF=5.862，II区）

刊号: 2012, 14 (13):3364-3367 

摘要: A PCR-based screening approach led to the identification of a deep-sea-derived Streptomyces sp. SCSIO 03032 capable of producing new bisindole alkaloids spiroindimicins A_D (1_4). Structural elucidation of these compounds revealed the presence of unusual [5,6] or [5,5] spiroring containing skeletons. Spiroindimicins B_D (2_4) with a [5,5] spiro-ring exhibited moderate cytotoxicities against several cancer cell lines. 

题目:  Carboxyl Formation from Methyl via Triple Hydroxylations by XiaM in Xiamycin A Biosynthesis 

作者: Qingbo Zhang, Huixian Li, Sumei Li, Yiguang Zhu, Guangtao Zhang, Haibo Zhang, Wenjun Zhang, Rong Shi, and Changsheng Zhang* 

刊物: Organic Letters,（IF=5.862，II区）

刊号: 2012, 14 (24): 6142–6145 

摘要: The P450 enzyme XiaM was identified as a candidate to form the C-24 carboxyl group in xiamycin A (1). Alteration of medium composition led to the discovery of four new compounds from the ΔxiaM and the ΔxiaK (encoding an aromatic ring hydroxylase) mutants. Biotransformation experiments revealed that XiaM was capable of converting a methyl group to a carboxyl group through diol and aldehyde intermediates.