题目:  Evaluation of sampling sizes on the intertidal macroinfauna assessment in a subtropical mudflat of Hong Kong 

作者: Shen Pingping, Zhou Hong, Zhao Zhenye, Yu Xiaozhang, Gu Jidong * 

刊物: Ecotoxicology ,（IF=2.355，II区）

刊号: 2012 (21): 1706-1716 

摘要: In this study, two types of sediment cores with different diameters were used to collect sediment samples from an intertidal mudflat in Hong Kong to investigate the influence of sampling unit on the quantitative assessment of benthic macroinfaunal communities. Both univariate and multivariate analyses were employed to detect differences in sampling efficiencies by the two samplers through total abundance and biomass, species richness and diversity, community structure, relative abundance of major taxa of the infaunal community. The species-area curves were further compared to find out the influence of the sampling units. Results showed that the two sampling devices provided similar information on the estimates of species diversity, density and species composition of the benthos in main part of the mudflat where the sediment was fine and homogenous; but at the station which contained coarse sand and gravels, the significant differences were detected between the quantitative assessments of macrobenthic infauna by the two samplers. Most importantly, the species-area curves indicated that more and smaller samples were better in capturing more species than less large ones when comparing an equal sampling area. Therefore, the efficiency of the sampler largely depended on the sediment properties, and sampling devices must be chosen based on the physical conditions and desired levels of precision on the organisms of the sampling program. 

题目: Novel polymorphism of internal transcribed spacers (ITS) and their utilization in phylogenetic analysis of Neanthes glandicincta (Annelida: Polychaeta: Nereididae)  

作者: Shen Pingping, Zhou Hong,  Gu Jiguang * 

刊物: Ecotoxicology ,（IF=2.355，II区）

刊号: 2012 (21): 1717-1725 

摘要: Sequences of internal transcribed spacers (ITS1 and ITS2) are increasingly being used to infer phylogenetic relationships at or below species levels. Here we report a novel case of ITS polymorphism within Neanthes glandicincta (Annelida: Polychaeta: Nereididae). Two types of ITS sequence (Type I and Type II) were cloned and sequenced, which showed significant differences both in nucleotide composition and length. Variations of these two types sequences also differed from each other with Type I was highly divergent while Type II was highly conserved. Phylogenetic trees inferred from ITS1 and ITS2 sequences showed striking discrepancy in N. glandicincta. Non-concerted evolution of multi-gene is suggested to be responsible for the high degree of polymorphism in ITS regions. Due to the two divergent types of ITS presented within a single N. glandicincta individual, the utilization of ITS regions for delineation of population or closely related species cannot be substantiated. The finding of different types of ITS in a single individual also stresses the need for analyzing a large number of clones whenever ITS sequences obtained by PCR amplification and cloning are being used in phylogenetic reconstruction. Keywords Neanthes glandicincta [1] Internal t