题目: Molecular cloning and characterization of class I NF-κB transcription factor from pearl oyster (Pinctada fucata) 

作者: Xian-De Huang, Mi Zhao, Wen-Guang Liu, Yun-Yan Guan, Yu Shi, Qi Wang, Shan-Zeng Wu, Mao-Xian He* 

刊物: Fish & Shellfish Immunology,（IF=3.628，III区）

刊号: 2012.38: 108-116 

摘要: NF-κB transcription factors play central roles in many important physiological and pathological processes including innate immune responses. Here we report the cloning of an NF-κB transcription factor, PfRelish from pearl oyster Pinctada fucata, one of the most important bivalve mollusks for seawater pearl production. PfRelish full-length cDNA is 3916 bp with an open reading frame of 3558 bp encoding a putative protein of 1186 amino acids. The deduced PfRelish contains a N-terminal RHD, a nucleus localization signal, an IκB-like domain with six ankyrin repeats and a death domain at the C-terminus, which is similar to class I NF-κB transcription factors. Comparison and phylogenetic analysis revealed that class I NF-κBs in mollusks including PfRelish might have most distant relationship to the arthropod Relish. Further expression analysis showed that PfRelish was apparently upregulated after Vibrio alginolyticus injection, which suggested that PfRelish was involved in the immune response to V. alginolyticus. 

题目:   Molecular cloning, characterization and expression analysis of Tumor necrosis factor receptor-associated factor 3 (TRAF3) from pearl oyster 

作者: Xian-De Huang, Mi Zhao, Wen-Guang Liu, Yun-Yan Guan, Yu Shi, Qi Wang, Shan-Zeng Wu, Mao-Xian He* 

刊物: Developmental and Comparative Immunology,（IF=3.322，I区）

刊号: 2012,33:652-658 

摘要: TRAF3 is a highly versatile regulator that negatively regulates JNK and alternative nuclear factor-κB signalling, but positively controls type I interferon production. To investigate TRAF3 function in innate immune responses among invertebrate especially mollusk, we characterized TRAF3 (PfTRAF3) from pearl oyster Pinctada fucata, one of the most important bivalve mollusks for seawater pearl production. PfTRAF3 cDNA is 2261 bp with an open reading frame of 1623 bp encoding a putative protein of 541 amino acids. The deduced PfTRAF3 contains a RING finger domain, two TRAF domains with zinc finger domains and a conserved C-terminal meprin and TRAF homology (MATH) domain. Comparison and phylogeneticanalysis revealed that PfTRAF3 from mollusk shared a higher identity with Ciona intestinalis TRAF3 from urochordata, Branchiostoma belcheri TRAF3 from cephalochordate, and even TRAF3 from vertebrate than with insect homologues. Furthermore, gene expression analyses suggested that PfTRAF3 was involved in the immune response to Vibrio alginolyticus.