题目: Insights into Caerulomycin A Biosynthesis:A Two-Component Monooxygenase CrmH-Catalyzed Oxime Formation 

作者: Yiguang Zhu, Qingbo Zhang, Sumei Li, Qinheng Lin, Peng Fu, Guangtao Zhang, Haibo Zhang, Rong Shi, Weiming Zhu, Changsheng Zhang* 

刊物: Journal of the American Chemical Society ,（IF=10.68，I区）

刊号:  2013,135:12152−12155 

摘要: The immunosuppressive agent caerulomycin A features a unique 2,2'-bipyridine core structure and an unusual oximefunctionality. Genetic and biochemical evidence confirms that the oxime formation in caerulomycin A biosynthesis is catalyzed by CrmH, a flavin-dependent two-component monooxygenase that is compatible with multiple flavin reductases, from a primary amine via a N-hydroxylamine intermediate. Structure homologue-guided site-directed mutagenesis studies identify four amino acid residues that are essential for CrmH catalysis. This study provides the first biochemical evidence of atwo-component monooxygenase that catalyzes oxime formation.