题目: Prauserella coralliicola sp. nov., isolated from the coral Galaxea fascicularis

作者:  Jiafa Wu, Jie Li*, Zhiqing You, Si Zhang*

刊物：International Journal of Systematic and Evolutionary Microbiology, (IF=2.798, III区)

刊号:  2014,64:3341-3345

摘要: A novel Gram-stain-positive actinobacterium, designated strain SCSIO 11529T, was isolated from tissues of the stony coral Galaxea fascicularis, and characterized by using a polyphasic approach. The temperature range for growth was 22–50 6C (optimum 28–45 6C), the pH range for growth was 6.0–8.0 (optimum pH 7.0), and the NaCl concentration range for growth was 0–7% (w/v) NaCl. The polar lipid profile contained diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and an unknown polar lipid. The predominant menaquinone was MK-9(H4). The major fatty acids (.10 %) were iso-C16 : 0, iso-C17 : 1v6c, iso-C16 : 1 H and C16 : 1v7c/iso-C15 : 0 2-OH. The DNA G+C content of strain SCSIO 11529T was 70.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCSIO 11529T belongs to the genus Prauserella, with the closest neighbours being Prauserella marina MS498T (97.0% 16S rRNA gene sequence limilarity), Prauserella rugosa DSM 43194T (96.4 %) and Prauserella flava YIM 90630T (95.9 %). Based on the evidence of the present study, strain SCSIO 11529T is considered to represent a novel species of the genus Prauserella, for which the name Prauserella coralliicola sp. nov. is proposed. The type strain is SCSIO 11529T (5DSM 45821T5NBRC 109418T).

题目: Mangrovibacteriumdiazotrophicum gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from a mangrove sediment, and proposal of Prolixibacteraceae fam. nov.

作者:  Xiaofang Huang, Yujuan Liu, Junde Dong, Lingyun Qu, Yanying Zhang, Fazuo Wang, Xinpeng Tian*, Si Zhang*

刊物：International Journal of Systematic and Evolutionary Microbiology, (IF=2.798, III区)

刊号:  2014,64:875-881

摘要: A nitrogen-fixing bacterium, designated strain SCSIO N0430T, was isolated from a mangrove sediment sample. Analysis of the sequence of the nifH gene responsible for nitrogen fixation in this strain indicated a close relationship to an uncultured bacterium ZNZ-D11 (GenBank accession no. JF896696). 16S rRNA gene sequence analysis revealed that this isolate had less than 93% similarity to its closest relative, Sunxiuqinia elliptica DQHS4T. A phylogenetic tree reconstructed based on 16S rRNA gene sequences revealed that strain SCSIO N0430T was a member of the phylum Bacteroidetes. Chemotaxonomic and physiological characteristics, including phospholipids and major fatty acids, readily distinguished the isolate from established members of the phylum Bacteroidetes. It is concluded that strain SCSIO N0430T represents a novel genus and species, for which the name Mangrovibacterium diazotrophicum gen. nov., sp.nov. is proposed, with the type strain of the species SCSIO N0430T (5KCTC 32129T5DSM 27148T5JCM 19152T). Based on phylogenetic characteristics and 16S rRNA gene signature nucleotide patterns, the three genera Sunxiuqinia, Prolixibacter and Mangrovibacterium are proposed to make up a novel family, Prolixibacteraceae fam. nov., in the order Bacteroidales.