题目: Activator Protein-1 (AP-1) and Response to Pathogen Infection in the Hong Kong Oyster (Crassostrea hongkongensis).

作者: Xiang, Z., F. Qu, J. Li, L. Qi, Y. Zhang, Z. Yu*

刊号: Fish & Shellfish Immunology, (IF-2.964, I区)

刊号: 2014,36:83-89

摘要: Growing evidence suggests that the transcription factor activator protein-1 (AP-1), a downstream target of mitogen-activated protein kinase (MAPK) signaling, plays a major role in stimulating the synthesis of immune effector molecules during innate immune responses. We have characterized ChAP-1, an AP-1-like protein in Crassostrea hongkongensis that is a member of the AP-1 family of proteins. ChAP-1 is composed of 290 amino acid residues with a Jun and bZIP domain at the N- and C-termini, respectively, a structure similar to that of known Ap-1 proteins. ChAP-1 mRNA is expressed in several tissues analyzed, with highest expression in the mantle. Expression of ChAP-1 increases in response to Vibrio alginolyticus, Salmo haemolyticus or Salmo cerevisiae infection and, despite the location of GFP-tagged full-length ChAP-1 protein in the cytoplasm, ChAP-1 activates the transcription of an L8G5-luc reporter gene, and its overexpression can also activate the AP-1-Luc reporter gene in HEK293T cells.

题目: A novel ortholog of serum response factor (SRF) with immune defense by a novel ortholog of serum response factor (SRF) in C. hongkongensis

作者:  Xiang, Z., F. Qu, L. Qi, Y. Zhang, S. Xiao, Z. Yu*

刊物：Fish & Shellfish Immunology (IF-2.964, I区)

刊号:  2014,36:75-82

摘要: Serum response factor (SRF) function is essential for transcriptional regulation of numerous growthfactor-inducible genes and triggers proliferation, differentiation and apoptosis of the cells. In this report, the first mollusk serum response factor like homolog gene (designated ChSRF) was identified and characterized from the Hong Kong oyster, Crassostrea hongkongensis. The full-length cDNA of ChSRF was 1716 bp in length and encodes a putative protein of 434 amino acids respectively, and shares the MADS domain at the N-terminal. ChSRF is ubiquitously expressed in various tissues, with the highest expression level observed in muscle. Temporal expression of ChSRF following microbe infection shows that the expression of ChSRF in hemocytes increases from 3 to 24 h post-challenge. As a target gene of SRF, b-actin demonstrates a similar gene expression mode in constitutive tissue and pathogen infection. Furthermore, some protein profiles of ChSRF was revealed, fluorescence microscopy results show that ChSRF located in the nuclei of HeLa cells and over-expression of ChSRF activated the transcriptional activities of MAPK signal pathway in HEK293T cells. These results indicate that ChSRF maybe play an important role in signal transduction in the immunity and development response of oysters. Furthermore, we found that ChSRF could regulate the expression of b-actin gene, which indicate that ChSRF is a muscle differentiation regulator in the oyster and it will help us to improve aquaculture production.