题目：DNA methylation is associated with expression level changes of galectin gene in mantle wound healing process of pearl oyster, Pinctada fucata

作者: Yaoguo Li, Xiande Huang, Yunyan Guan, Yu Shi, Hua Zhang, Maoxian He*

刊物: Fish & Shellfish Immunology（IF=2.674，农林科学I区）

刊号:(2015)45:912-918

摘要: Galectin is one important member of pattern recognition proteins that plays a pivotal role in regulating innate immune response of invertebrates. In this study, we cloned the promoter sequence of a tandemrepeat galectin from the pearl oyster Pinctada fucata (P. fucata). The quantitative real-time PCR analysis revealed that galectin mRNA expression in mantle tissues were firstly up-regulated from time points of 2 he24 h, and then down-regulated from time points of 24 he168 h after mantle injury. The genome methylation level of mantle tissue was inversely related to galectin mRNA expression (Pearson correlation: _0.554, P: 0.154). The “CpG4-6” methylation level in promoter region of galectin was significant positive correlated with the mRNA expression (Pearson correlation: 0.313, P: 0.049). The results indicated that galectin gene may be involved in immune response in mantle wound healing process of P. fucata, and DNA methylation may be a regulation factor of gene expression.

关键词: Pinctada fucata，Mantle injury，Galectin，Promoter region，DNA methylation

题目：Nuclear factor of activated T cells (NFAT) in pearl oyster Pinctada fucata: Molecular cloning and functional characterization

作者: Xian-de Huang, Guo-jian Wei, Hua Zhang, Mao-xian He*

刊物: Fish & Shellfish Immunology（IF=2.674，农林科学I区）

刊号: (2015) 42:108-113

摘要: Nuclear factor of activated T cells (NFAT) plays an important role in nonimmune cells and also in T cells and many other cells of the immune system, by regulating the expression of a variety of genes involved in the immune response, organ development, developmental apoptosis and angiogenesis. In the present study, the NFAT homology gene, PfNFAT, from the pearl oyster Pinctada fucatawas cloned and its genomic structure and promoter were analyzed. PfNFAT encodes a putative protein of 1226 amino acids, and contains a highly conserved Rel homology region (RHR) with DNA-binding specificity, and a regulatory domain (NFAT homology region, NHR) containing a potent transactivation domain (TAD). The PfNFAT gene consists of 12 exons and 11 introns, and its promoter contains potential binding sites for transcription factors such as NF-kB (Nuclear factor kB), STATx (signal transducer and activator of transcription), AP-1 (activator protein-1) and Sox-5/9 (SRY type HMG box-5/9), MyoD (Myogenic Differentiation Antigen) and IRF (Interferon regulatory factor). Comparison and phylogenetic analysis revealed that PfNFAT shows high identity with other invertebrate NFAT, and clusters with the NFAT5 subgroup. Furthermore, gene expression analysis revealed that PfNFAT is involved in the immune response to lipopolysaccharide (LPS) and Polyinosinic-polycytidylic acid (poly I:C) stimulation and in the nucleus inserting operation. The study of PfNFAT may increase understanding of molluscan innate immunity.

关键词: Pearl oyster, PfNFAT, Gene cloning, Gene expression

题目：Cloning and gene expression of signal transducers and activators of transcription(STAT) homologue provide new insights into the immune response and nucleus graft of the pearl oyster Pinctada fucata

作者: Xian-De Huang, Guo-Jian Wei, Mao-Xian He*

刊物: Fish & Shellfish Immunology（IF=2.674，农林科学I区）

刊号: (2015) 47:847-854

摘要: The signal transducers and activators of the transcription (STAT) family play an important role in regulatory and cellular functions by regulating the expression of a variety of genes, including cytokines and growth factors. In the present study, a Pinctada fucata STAT protein, termed PfSTAT, was described. The deduced amino acid sequence of PfSTAT contains the conserved STAT_bind domain and the SH2 domain, and the additional Bin/Amphiphysin/Rvs (BAR) domain, but does not have STAT_alpha and STAT_int domains. Multiple sequence alignments revealed that PfSTAT showed relatively low identity with vertebrate and other invertebrate STATs, and phylogenetic analysis indicated that the evolution of STAT may have been more complex and ancient. Gene expression analysis revealed that PfSTAT is involved in the immune response to polyinosinicepolycytidylic acid (poly I:C) stimulation and in the nucleus insertion operation. This study contributes to a better understanding of PfSTAT in protecting the pearl oyster from disease or injury caused by grafting.

关键词: Pearl oyster, PfSTAT, Immunity, Graft, Gene expression