题目: Exploring fungal diversity in deep-sea sediments from Okinawa through using high-throughput illumina sequencing

作者: Xiaoyong Zhang, Guanghua Wang, Xinya Xu, Xuhua Nong, Jie Wang, Muhammad Amin, Shuhua Qi*

刊物: Deep-Sea Research I

年卷期页: 2016,116: 99-105

摘要: The present study investigated the fungal diversity in four different deep-seas ediments from Okinawa Trough using high-through put Illumina sequencing of the nuclear ribosomal internal transcribed spacer- 1 (ITS1). At otal of 40,297 fungal ITS1 sequence sclustered into 420 operational taxonomicunits(OTUs) with 97% sequence similarity and 170 taxawerere covered from these sediments. Most ITS1 sequences (78%)  belonged to the phylum Ascomycota, followed by Basidiomycota (17.3%), Zygomycota (1.5%) and Chytridiomycota (0.8%), and a small proportion (2.4%) belonged to unassigned fungal phyla.Compared with previous studies on fungal diversity of sediments from deep-sea environments by culture-depen- dent approach and clonelibrary analysis, the present result suggested that Illumina sequencing had been dramatically accelerating the discovery off ungal community of deep-sea sediments.Further more,our results revealed that Sordariomycetes was the most diverse and abundant fungal classinthis study, challenging the traditional view that the diversity of Sordario mycetesphylo types was low in the deep- sea environments. In addition, more than 12 taxa accounted for 21.5% sequences were found to be rarely reported as deep-sea fungi, suggesting the deep-sea sediments from OkinawaTrough harbored aple- thora of different fungal communities compared with other deep-sea environments. To our knowledge, this study is the first exploration of the fungal diversity in deep-sea sediments from OkinawaTrough using high-through put Illumina sequencing.