题目:Pyomelanin fromPseudoalteromonas lipolyticareduces biofouling

作者:Zhenshun Zeng+, Xing-Pan Guo+, Xingsheng Cai, Pengxia Wang, Baiyuan Li, Jin-Long Yang* and Xiaoxue Wang*

刊物:Microbial biotechnology

年卷期页: 2017,10(6):1718-1731

摘要：Members of the marine bacterial genus Pseudoalteromonas are efficient producers of antifouling agents that exert inhibitory effects on the settlement of invertebrate larvae. The production of pigmented secondary metabolites byPseudoalteromonashas been suggested to play a role in surface colonization. However, the physiological characteristics of the pigments produced by Pseudoalteromonas remain largely unknown. In this study, we identified and characterized a genetic variant that hyperproduces a dark-brown pigment and was generated during Pseudoalteromonaslipolytica biofilm formation. Through whole-genome resequencing combined with targeted gene deletion and complementation, we found that a point mutation within thehmgAgene, which encodes homogentisate 1,2-dioxygenase, is solely responsible for the overproduction of the dark-brown pigment pyomelanin. InP. lipolytica, inactivation of thehmgAgene led to the formation of extracellular pyomelanin and greatly reduced larval settlement and metamorphosis of the musselMytiluscoruscus. Additionally, the extracted pyomelanin from thehmgAdeletion mutant and the invitrosynthesizedpyomelanin also reduced larval settlement and metamorphosis ofM. coruscus, suggesting that extracellularpyomelaninreleased from marine Pseudoalteromonas biofilm can inhibit the settlement of fouling organisms.