您的位置:首页>论文发表>2020
2020

喻子牛研究团队在《Frontiers in immunology》发表论文

副标题:

时间:2020-06-24  作者:LMB  来源:文本大小:【 |  | 】  【打印

题目: Phagocyte Transcriptomic Analysis Reveals Focal Adhesion Kinase (FAK) and Heparan Sulfate Proteoglycans (HSPGs) as Major Regulators in Anti-bacterial Defense of Crassostrea hongkongensis

作者: Yue Lin, Fan Mao, Nai-Kei Wong, Xiangyu Zhang, Kunna Liu, Minwei Huang, Haitao Ma, Zhiming Xiang, Jun Li, Shu Xiao, Yang Zhang, Ziniu Yu

刊物: Frontiers in immunology

年卷期页: 2020, 11: 416

摘要Invertebrates generally lack adaptive immunity and compensate for this with highly efficient innate immune machineries such as phagocytosis by hemocytes to eradicate invading pathogens. However, how extrinsically cued hemocytes marshal internal signals to accomplish phagocytosis is not yet fully understood. To this end, we established a facile magnetic cell sorting method to enrich professional phagocytes from hemocytes of the Hong Kong oyster (Crassostrea hongkongensis), an ecologically and commercially valuable marine invertebrate. Transcriptomic analysis on presorted cells shows that phagocytes maintain a remarkable array of differentially expressed genes that distinguish them from non-phagocytes, including 352 significantly upregulated genes and 479 downregulated genes. Pathway annotations reveal that focal adhesion and extracellular matrix-receptor interactions were the most conspicuously enriched pathways in phagocytes. Phagocytosis rate dramatically declined in the presence of an FAK inhibitor, confirming importance of the focal adhesion pathway in regulating phagocytosis. In addition, we also found that heparan sulfate proteoglycan (HSPG) families were lineage-specifically expanded in C. hongkongensis and abundantly expressed in phagocytes. Efficiency of phagocytosis and hemocytes aggregation was markedly reduced upon blockage of endogenous synthesis of HSPGs, thus implicating these proteins as key surface receptors in pathogen recognition and initiation of phagocytosis.

相关附件
相关文档
Copyright    中国科学院热带海洋生物资源与生态重点实验室    版权所有
地址:广东省广州市新港西路164号  邮编:510301   粤ICP备05007992号    粤公网安备44011502001245号
电话:020-89023101  传真:86-20-84451672  Email:liujuan@scsio.ac.cn