题目: Characterization of tailoring methyltransferases involved in K-41A biosynthesis: modulating methylation to improve K-41A anti-infective activity

作者: Chen Jiang, Gui Chun, Wei Qiuyu, Liu Jie, Ye Li, Tian Xinpeng, Gu Yu-Cheng, Li Qinglian, Ju Jianhua

刊物: Organic Letters

年卷期页: 2020, 22: 4627-4632

摘要：The biosynthetic gene cluster (BGC) for polyether antibiotic K-41A was identified from marine-derived Streptomyces sp. SCSIO 01680 and subjected to combinatorial biosynthetic study. Bioinformatics analyses, gene disruption, and metabolomics analyses afforded eight new derivatives and one known polyether, showcasing five region-specific methyltransferases Pak13, Pak15, Pak20, Pak31, and Pak38 and their respective modification loci. Moreover, bioassays revealed that two disaccharide-bearing polyethers, K-41B and K-41Bm, display enhanced anti-HIV and potent antibacterial activities.