王晓雪研究员

中国科学院热带海洋生物资源与生态重点实验室 主任

（海洋微生物被膜学科组责任研究员  E-mail：xxwang@scsio.ac.cn）

研究领域  

      主要从事海洋原核生物的环境适应与进化机制研究，聚焦宿主与噬菌体的互作机制和毒素-抗毒素的多样性与功能研究。

代表性工作简介

系统性研究发现温和噬菌体调控海洋细菌对特殊生境适应性进化和生态功能。在海洋毒素-抗毒素系统（TA）领域取得了一系列引领性原创成果，发现两大全新类型的TA系统，揭示TA系统是温和噬菌体与海洋细菌建立“互利共生”关系的核心。在海洋生物资源保护和应用领域做出重要的贡献。以通讯或第一作者在Nature Ecology & Evolution, Nature Chemical Biology, PNAS, Nucleic Acids Research等权威期刊发表论文50余篇。论文多次被Faculty of 1000推荐评论。授权发明专利4项。

工作经历

2006.12 - 2007.12  美国Rutgers University 博士后

2008.04 - 2011.11  美国Texas A&M University博士后

2011.12 - 至今      中国科学院南海海洋研究所 研究员

2017.02 - 2017.05  哈佛医学院 高级访问学者

教育背景

1996.09 - 2000.07 中国海洋大学 食品工程 本科学位

2002.05 - 2006.12 美国Texas A&M University 遗传学 博士学位

获奖及荣誉

2022年度中国科学院大学院级研究生优秀课程《生物有机化学》

2023年度中国科学院优秀导师奖

代表性成果

1. Wang W^#, Tang K^#, Wang P^#, Zeng ZS, Xu T, Zhan WE, Liu TL, Wang Y, Wang X*. (2022). The coral pathogen Vibrio coralliilyticus kills non-pathogenic holobiont competitors by triggering prophage induction. Nature Ecology & Evolution. 6, 1132–1144

2. Wang PX, Zhao Y, Wang WQ, Lin ST, Tang KH, Liu TL, Wood TK, Wang X*. (2022). Mobile genetic elements used by competing coral microbial populations increase genomic plasticity. ISME Journal. 16, 2220–2229

3. Ni S, Li B, Tang K, Yao J, Wood TK, Wang P*, Wang X*. (2021) Conjugative plasmid-encoded toxin-antitoxin system PrpT/PrpA directly controls plasmid copy number. Proc. Natl. Acad. Sci. USA.118 (4) e2011577118.

4.  Wang X*, Yao J, Sun Y, Wood TK*. (2021) Type VII toxin/antitoxin classification system for antitoxins that enzymatically neutralize toxins. Trends in Microbiology 29(5): 388-393.

5. Tang K, Wang W, Sun Y, Zhou Y, Wang P, Guo Y, Wang X*. (2021) Prophage Tracer: precisely tracing prophages in prokaryotic genomes using overlapping split-read alignment. Nucleic Acids Research 16; 49(22): e128.

6. Liu X, Lin S, Liu T, Zhou Y, Wang W, Yao J, Guo Y, Tang K, Chen R, Benedik MJ, Wang X*. (2021) Xenogeneic silencing relies on temperature dependent phosphorylation of the host H-NS protein in Shewanella. Nucleic Acids Research 49(6): 3427-3440.

7. Yao J, Zhen X, Tang K, Liu T, Xu X, Chen Z, Guo Y, Liu X, Wood TK, Ouyang S*, Wang X*. (2020) Novel polyadenylylation-dependent neutralization mechanism of the HEPN/MNT toxin/ antitoxin system. Nucleic Acids Research 48(19): 11054–11067.

8. Song S, Guo Y, Kim JB, Wang X*, Wood TK*. (2019) Phage mediate bacterial self-recognition. Cell Reports. 27 (3), 737-749.-7495

9.  Li Y, Liu X, Tang K, Wang P, Zeng Z, Guo Y, Wang X*. (2019) Excisionase in Pf filamentous prophage controls lytic-lysogeny decision-making in Pseudomonas aeruginosa. Molecular Microbiology 111(2), 495–513.

10.Yao J, Guo Y, Wang P, Zeng Z, Li B, Tang K, Liu X, Wang X*. (2018) Type II toxin/antitoxin system ParE_SO/CopA_SO stabilizes prophage CP4So in Shewanella oneidensis. Environmental Microbiology. 20, 1224-1239.

11. Guo Y, Liu X, Li B, Yao J, Wood TK, Wang X*. (2017) Tail-anchored inner membrane protein ElaB increases resistance to stress while reducing persistence in Escherichia coli. Journal of Bacteriology 199: e00057-17.

12. Zeng Z, Liu X, Yao J, Guo Y, Li B, Li Y, Jiao N, Wang X*. (2016) Cold adaptation regulated by cryptic prophage excision in Shewanella oneidensis. ISME Journal 10(12):2787-2800.

13.  Wang X*, Wood TK* (2016). Cryptic prophages as targets for drug development. Drug Resistance Updates 27:30-8.

14.  Guo Y, Quiroga C, Chen Q, McAnulty MJ, Benedik MJ, Wood TK*, Wang X*. (2014) RalR (a DNase) and RalA (a small RNA) form a type I toxin–antitoxin system in Escherichia coli. Nucleic Acids Research 42 (10): 6448-6462.

15.  Wang X^#, Lord DM^#, Cheng HY^#, Osbourne DO, Hong SH, Sanchez-Torres V, Quiroga C, Zheng K, Herrmann T, Peti W, Benedik MJ, Page R*, Wood TK*. (2012) A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS. Nature Chemical Biology 8(10):855-61. (# co-first authors).

16.  Wang X, Kim Y, Hong SH, Ma Q, Brown BL, Pu M, Tarone AM, Benedik MJ, Peti W, Page R, Wood TK*. (2011) Antitoxin MqsA helps mediate the bacterial general stress response. Nature Chemical Biology 7, 359-366.

17.  Wang X, Wood TK*. (2011) Toxin/antitoxin systems influence biofilm and persister cell formation and the general stress response. Applied & Environmental Microbiology 77, 5577-5583.

18.  Wang X, Wood TK*. (2011) IS5 inserts upstream of flhDC in a quasi-Lamarckian way. ISME Journal 5, 1517–1525.

19.  Wang X, Kim Y, Ma Q, Hong S-H, Pokusaeva K, Sturnio JM, Wood TK*. (2010) Cryptic prophages help bacteria cope with adverse environments. Nature Communications 1, 147.

20.  Wang X, Kim Y, Wood TK*. (2009) Control and benefits of CP4-57 prophage excision in Escherichia coli biofilms. ISME Journal 3, 1164-1179.

专利

1.   一种生物膜基因岛 GIVal43097 及其切除方法；王晓雪，王鹏霞，王伟权，汤开浩；发明专利，授权专利号：ZL202010929895.2，授权日期：2022.09.20

2.   一种快速定量 ssDNA 丝状噬菌体的方法及其应用；王晓雪，郭云学，林兼仲，汤开浩，高欣宇，古嘉瑜；发明专利，授权专利号：ZL202210137549.X，授 权日期：2022.02.15

3.   一种调控珊瑚菌群的温和噬菌体 VneM1 及其应用；王晓雪，王伟权，王鹏霞，汤开浩.发明专利, 授权专利号：ZL202011194541.4, 授权日期：2021.01.19.

4.  一种高效热稳定的核糖核酸酶 SiRe_0917 及其编码基因和应用，姚建云，王晓雪，冯旭，陈喆；发明专利，专利号 ZL202310191991.5，授权时间 2023.11.14

培养研究生情况

在读博士研究生

2019级：陈喆（直博）

2020级：刘自尧，刘天朗

2021级：林兼仲（直博）

2022级：林世团，古嘉瑜，严嘉慧（中国地质大学（北京）联合培养）

在读硕士研究生

2021级：高欣宇

2022级：刘心雨

2023级：费星怡

已培养博士6名，硕士4名。