张长生研究员

中国科学院南海海洋研究所 副所长

中国科学院中国-斯里兰卡联合科教中心（中-斯中心） 主任

（海洋微生物代谢工程与生物合成学科组组长 czhang@scsio.ac.cn）

研究兴趣与领域

1. 微生物天然活性物质的分离鉴定

2. 复杂微生物次生代谢产物的生物合成研究

3. 微生物次生代谢产物新功能酶的作用机制及其应用

代表性工作简介

主要从事海洋微生物代谢工程研究，致力于从海洋微生物中发现、分离和鉴定具有抗感染、抗肿瘤等活性的天然物质，并进行组合生物合成研究，用代谢工程手段改造和优化其结构与活性，为发现和研制海洋微生物药物先导化合物奠定物质基础。在海洋放线菌次级代谢产物的分离鉴定和活性评价、海洋微生物代谢产物的环化代谢工程、糖苷类药物的糖基化代谢工程研究领域做出了杰出贡献。

学习经历

1990.08-1994.07：上海交通大学生物科学与技术专业，学士

1994.09-1997.07：华东理工大学生物化工专业，硕士

1998.09-2002.12：Wuppertal University, Chemical Microbiology，博士

工作经历

1997.07-1998.08：上海太平洋生物高科技有限公司，基因工程，助理研究员

2003.01-2006.06：美国威斯康星大学-麦迪逊分校药学院，博士后

2006.07-2008.03：美国威斯康星大学-麦迪逊分校药学院，Assistant Scientist

2008.03-至今：中国科学院南海海洋研究所，责任研究员

获奖与荣誉

1. 国家杰出青年基金获得者（2011）

2. 第五届全国优秀科技工作者（2012）

3. 科技部中青年科技创新领军人才（2014）

4. 百千万工程国家级人选“有突出贡献中青年专家”（2014）

5. 广东省特支计划“百千万工程领军人才”（2014）

6. 中科院特聘骨干人才研究员（2015）

7. 广东省特支计划“杰出人才”（南粤百杰）（2015）

8. 国家高层次领军人才（2016）

9. 国务院特殊津贴获得者（2017）

10. 中国科学院院长特别奖（导师奖）（2018）

学术兼职

1. 中国生物化学与分子生物学海洋生物化学与分子生物学分会副理事长（2016-2020）2. 中国海洋湖沼学会药学分会副理事长（2017-2022）

3. 中国药学会海洋药物专业委员会委员（2019-2024）

4. 中国微生物学会分子微生物学与生物工程专业委员会委员（2016-2021）

5. 中国微生物学会海洋微生物学专业委员会委员（2016-2021）

6. 中国科学院青年创新促进会广州分会顾问委员会委员（2016-2020）

7. 中国海洋学会热带海洋分会委员（2019-2022）

8. 广东省第九届海洋湖沼学会理事（2019-2024）

9. 广东省第八届海洋学会理事（2019-2024）

10 .广东省药学会药物化学专业委员会委员（2018-2021）

11.《热带海洋学报》副主编（2014-至今）

12.《中国海洋药物》编委（2013-至今）

13.《微生物学报》编委（2017-至今）

14. Natural Product Reports 编委（Editorial Board）（2020-至今）

15. Molecules 编委（Editorial Board）（2020-至今）

代表性学术成果

论文发表

2021

78. Chunfang Yang⁺, Chunshuai Huang⁺, Chunyan Fang, Liping Zhang, Siqiang Chen, ingbo Zhang, Changsheng Zhang*, Wenjun Zhang*. Inactivation of flavoenzyme-encoding gene flsO1 in fluostatin biosynthesis leads to diversified angucyclinone derivatives. The Journal of Organic Chemistry, 2021, DOI: 10.1021/acs.joc.0c02517.

77. Xinya Zhang⁺, Siqiang Chen⁺, Liping Zhang, Qingbo Zhang, Wenjun Zhang, Yuchan Chen, Weimin Zhang, Haibo Zhang*, and Changsheng Zhang*. Dassonmycins A and B, Polycyclic Thioalkaloids from a Marine Sponge-Derived Nocardiopsis dassonvillei SCSIO 40065. Organic Letters. 2021. https://doi.org/10.1021/acs.orglett.1c00328

2020

76. Liping Zhang⁺, Bidhan Chandra De⁺, Wenjun Zhang*, Attila Mándi,  Zhuangjie Fang,  Chunfang Yang,  Yiguang Zhu,  Tibor Kurtán,  and Changsheng Zhang*. Mutation of an atypical oxirane oxyanion hole improves regioselectivity of the α/β-fold epoxide hydrolase Alp1U. Journal of Biological Chemistry. 2020, 295(50): 16987-16997

75. Chunyan Fang,  Qingbo Zhang,  Yiguang Zhu,  Liping Zhang,  Wenjun Zhang,  Liang Ma,  Haibo Zhang,  and  Changsheng Zhang*. Proximicins F and G and diproximicin A: aminofurans from the marine-derived Verrucosispora sp. SCSIO 40062 by overexpression of PPtase genes. Journal of Natural Products. 2020, 83(4): 1152-1156

74. Zhuangjie Fang⁺, Xiaodong Jiang⁺, Qingbo Zhang*, Liping Zhang,  Wenjun Zhang,  Chunfang Yang,  Haibo Zhang,  Yiguang Zhu, Changsheng Zhang*. S-Bridged thioether and structure-diversified angucyclinone derivatives from the South China Sea-derived Micromonospora echinospora SCSIO 04089. Journal of Natural Products. 2020, 83(10): 3122-3130

73. Imran Khan, Haibo Zhang*, Wei Liu,  Liping Zhang, Fang Peng, Yuchan Chen, Qingbo Zhang, Guangtao Zhang, Weimin Zhang, and Changsheng Zhang*. Identification and bioactivity evaluation of secondary metabolites from Antarctic-derived Penicillium chrysogenum CCTCC M 2020019. RSC Advances. 2020, 10(35): 20738-20744

72. Yiguang Zhu⁺, Qingbo Zhang⁺, Chunyan Fang, Yingli Zhang, Liang Ma, Zhiwen Liu, Shilan Zhai, Jing Peng, Liping Zhang, Weiming Zhu*, Changsheng Zhang*. Refactoring the concise biosynthetic pathway of cyanogramide unveils a P450 enzyme-catalyzed spirooxindole formation. Angewandte Chemie International Edition, 2020, 59: 14065-14069

71. Wei Liu⁺, Vinay Gopal Jannu⁺, Zhiwen Liu, Qingbo Zhang, Xiaodong Jiang, Liang Ma, Wenjun Zhang, Changsheng Zhang*, Yiguang Zhu.* Heterologous expression of the trichostatin genecluster and functional characterization of the N-methyltransferase TsnB8. Organic & Biomolecular  Chemistry, 2020, 18(19): 3649-3653 

70. Bin Tan⁺, Qingbo Zhang⁺, Yiguang Zhu, Hongbo Jin, Liping Zhang, Siqiang Chen, and Changsheng Zhang*. Deciphering Biosynthetic Enzymes Leading to 4-Chloro-6-Methyl-5,7-Dihydroxyphenylglycine, a Non-Proteinogenic Amino Acid in Totopotensamides, ACS chemical biology. 2020, 15(3): 766-773

 69. Hongbo Jin, Wenjun Zhang, Guangtao Zhang, Liping Zhang, Wei Liu, and Changsheng Zhang*, Engineered Biosynthesis of 5/5/6 Type Polycyclic Tetramate Macrolactams in an Ikarugamycin (5/6/5 Type)-Producing Chassis. Organic letters.  2020. 2020, 22, 5, 1731-1735

68. Bin Tan⁺, Siqiang Chen⁺, Qingbo Zhang^*, Yuchan Chen, Yiguang Zhu, Imran Khan, Weimin Zhang, and Changsheng Zhang*. Heterologous Expression Leads to Discovery of Diversi？ed Lobophorin Analogues and a Flexible Glycosyltransferase. Org. Letts.2020, 22(3): 1062-1066

2019

67. Zhiwen Liu⁺, Liang Ma⁺, Liping Zhang, Wenjun Zhang, Yiguang Zhu, Yuchan Chen, Weimin Zhang, Changsheng Zhang*. Functional characterization of the halogenase SpmH and discovery of new deschloro-tryptophan dimers. Organic & biomolecular chemistry. 2019, 17(2): 1053-1057.

66. Chunshuai Huang⁺, Chunfang Yang⁺, Zhuangjie Fang, Liping Zhang, Wenjun Zhang, Yiguang Zhu, Changsheng Zhang*. Discovery of Stealthin Derivatives and Implication of the Amidotransferase FlsN3 in the Biosynthesis of Nitrogen-Containing Fluostatins. Marine drugs. 2019, 17(3): 150.

65. Chunshuai Huang, Chunfang Yang, Wenjun Zhang, Yiguang Zhu, Liang Ma, Zhuangjie Fang, Changsheng Zhang*. Albumycin, a new isoindolequinone from Streptomyces albus J1074 harboring the fluostatin biosynthetic gene cluster. The Journal of antibiotics. 2019, 72(5): 311.

64. Ziquan Yu, Haibo Zhang, Chengshan Yuan, Qingbo Zhang, Imran Khan, Yiguang Zhu, Changsheng Zhang*. Characterizing two cytochrome P450s in tiacumicin biosynthesis reveals reaction timing for tailoring modifications. Organic Letters. 2019, 21(18): 7679-7683

63. Liujuan Zheng⁺, Xiaodong Jiang⁺, Qingbo Zhang, Yiguang Zhu, Haibo Zhang, Wenjun Zhang, Kumar Saurav, Jinsong Liu, Changsheng Zhang*. Discovery and biosynthesis of neoenterocins indicate a skeleton rearrangement of enterocin. Organic Letters. 2019, 21(22): 9066-9070

62. Wei Liu, Wenjun Zhang, Hongbo Jin, Qingbo Zhang, Yuchan Chen, Xiaodong Jiang, Guangtao Zhang, Liping Zhang, Weimin Zhang, Zhigang She, Changsheng Zhang*. Genome mining of marine-derived Streptomyces sp. SCSIO 40010 leads to cytotoxic new polycyclic tetramate macrolactams. Marine Drugs. 2019, 17(12): 663

61. Zhuangjie Fang, Siqiang Chen, Yiguang Zhu, Jie Li, Imran Khan, Qingbo Zhang*, Changsheng Zhang*. A new uridine derivative and a new indole derivative from the coral-associated actinomycete Pseudonocardia sp. SCSIO 11457. Natural Product Research. 2019, DOI: 10.1080/14786419.2019.1616729

2018

60. Chunshuai Huang⁺, Chunfang Yang⁺, Wenjun Zhang, Liping Zhang, Bidhan Chandra De, Yiguang Zhu, Xiaodong Jiang, Chunyan Fang, Qingbo Zhang, Cheng-Shan Yuan, Hung-wen Liu* & Changsheng Zhang*. Molecular Basis of Dimer Formation during the Biosynthesis of Benzofluorene-Containing Atypical Angucyclines. Nature Communications. 2018, 9: 2088-2097.

59. Chunshuai Huang, Chunfang Yang, Yiguang Zhu*, Wenjun Zhang, Chengshan Yuan and Changsheng Zhang*. Marine Bacterial Aromatic Polyketides From Host-Dependent Heterologous Expression and Fungal Mode of Cyclization. Frontiers in Chemistry. 2018, 6: 528-525.

58. Haibo Zhang, Xiaoxing Tian Xiaohui Pu, Qingbo Zhang, Wenjun Zhang, and Changsheng Zhang*. Tiacumicin Congeners with Improved Antibacterial Activity from a Halogenase-Inactivated Mutant. Journal of Natural Products. 2018, 81: 1219-1224.

57. Wenjun Zhang, Guangtao Zhang, Liping Zhang, Wei Liu, Xiaodong Jiang, Hongbo Jin, Zhiwen Liu, Haibo Zhang, Aihua Zhou, Changsheng Zhang*. New polycyclic tetramate macrolactams from marine-derived Streptomyces sp. SCSIO 40060. Tetrahedron. 2018, 74(47): 6839-6845.

2017

56. Subhasish Saha⁺, Wenjun Zhang⁺, Guangtao Zhang⁺, Yiguang Zhu, Yuchan Chen, Qingbo Zhang, Haibo Zhang, Liping Zhang, Weimin Zhang and Changsheng Zhang*. Activation and characterization of a cryptic gene cluster reveals a cyclization cascade for polycyclic tetramate macrolactams. Chemical Science. 2017, 8: 1607-1612.

55. Qingbo Zhang⁺, Huixian Li⁺, Lu Yu, Yu Sun, Yiguang Zhu, Hanning Zhu, Liping Zhang, Shu-Ming Li, Yuemao Shen, Changlin Tian, Ang Li, Hung-wen Liu* and Changsheng Zhang*. Characterization of the Flavoenzyme XiaK as an N-Hydroxylase and Implications in Indolosesquiterpene Diversification. Chemical Science. 2017, 8(7): 5067-5077.

54. Wenjun Zhang⁺, Chunfang Yang⁺, Chunshuai Huang, Liping Zhang, Haibo Zhang, Qingbo Zhang, Chengshan Yuan, Yiguang Zhu, and Changsheng Zhang*. Pyrazolofluostatins A–C, Pyrazole-Fused Benzo[a]fluorenes from South China Sea-Derived Micromonospora rosaria SCSIO N160. Organic Letters.

53. Ruidong Chen⁺, Qingbo Zhang⁺, Bin Tan, Liujuan Zheng, Huixian Li, Yiguang Zhu, and Changsheng Zhang*. Genome Mining and Activation of a Silent PKS/NRPS Gene Cluster Direct the Production of Totopotensamides. Organic Letters. 2017, 19(20): 5697-5700.

52. Liang Ma, Wenjun Zhang, Yiguang Zhu, Guangtao Zhang, Haibo Zhang, Qingbo Zhang, Liping Zhang, Chengshan Yuan, Changsheng Zhang*. Identification and Characterization of a Biosynthetic Gene Cluster for Tryptophan Dimers in Deep Sea-Derived Streptomyces sp. SCSIO 03032. Applied Microbiology and Biotechnology. 2017, 101(15): 6123-6136.

51. Xiaodong Jiang, Qingbo zhang, Yiguagn Zhu, Fangling Nie, Zhengchao Wu, Chunfang Yang, Liping Zhang, Xinpeng Tian, ChangshengZhang*. Isolation, structure elucidation and biosynthesis of benzo[b]fluorene nenestatin A from deep-sea derived Micromonospora echinospora SCSIO 04089. Tetrahedron. 2017, 73(26): 3585-3590.

2016

50. Zhu Yiguang⁺, Picard Mè⁺, Zhang Qingbo, Barma J, Després X, Mei Xiangui, Zhang Liping, Duvignaud J, Couture M, Zhu Weiming, Shi Rong*, Zhang Changsheng*. Flavoenzyme CrmK-Mediated Substrate Recycling in Caerulomycin Biosynthesis. Chemical Science. 2016, 7(8), 4867-4874.

49. Zhu Yiguang⁺, Xu Jinxin⁺, Mei Xiangui⁺, Feng Zhan, Zhang Liping, Zhang Qingbo, Zhang Guangtao, Zhu Weiming*, Liu Jinsong*, Zhang Changsheng*. Biochemical and Structural Insights into the Aminotransferase CrmG in Caerulomycin Biosynthesis. ACS Chemical Biology. 2016, 11(4): 943-952.

48. Zhang Guangtao, Zhang Wenjun, Saha Subhasish, Zhang Changsheng*. Recent Advances in Discovery, Biosynthesis and Genome Mining of MedicinallyRelevant Polycyclic Tetramate Macrolactams. Current Topics in Medicinal Chemistry. 2016, 16(15): 1727-1739.

47. Zhang Haibo⁺, Saurav Kumar⁺, Yu Ziquan, Tibor Kurtan, Mándi Attila, Li Jie, Tian Xinpeng, Zhang Qingbo, Zhang Wenjun, Zhang Changsheng*. α-Pyrones with Diverse Hydroxy Substitutions from Three Marine-Derived Nocardiopsis Strains. Journal of Natural Products. 2016, 79(6): 1610-1618.

2015

46. Huixian Li⁺, Yu Sun⁺, Qingbo Zhang, Yiguang Zhu, Sumei Li, Ang Li, Changsheng Zhang*. Elucidating the cyclization cascades in xiamycin biosynthesis by substrate synthesis and enzyme characterizations. Organic Letters. 2015, 17(2): 306-309.

45. Chunfang Yang, Chunshuai Huang,Wenjun Zhang*,Yiguang Zhu ,Changsheng Zhang*. Heterologous Expression of Fluostatin Gene Cluster Leads to a Bioactive Heterodimer. Organic Letters. 2015, 17(21): 5324-5327.

44. Yiguang Zhu⁺, Wenjun Zhang⁺, Yaolong Chen, Chengshan Yuan, Haibo Zhang, Guangtao Zhang, Liang Ma, Qingbo Zhang, Xinpeng Tian, Si Zhang, Changsheng Zhang*. Characterization of Heronamide Biosynthesis Reveals A Tailoring Hydroxylase and Indicates Migrated Double Bonds. ChemBioChem. 2015, 16(14): 2086-2093.

2014

43. Zhang Guangtao⁺, Zhang Wenjun⁺, Zhang Qingbo, Shi Ting, Ma Liang, Zhu Yiguang, Li Sumei, Zhang Haibo, Zhao Yilei, Shi Rong, Zhang Changsheng*. Mechanistic Insights Into Polycycle Formation by Reductive Cyclization in Ikarugamycin Biosynthesis. Angewandte Chemie International Edition. 2014, 53(19): 4840-4844.

42. Peng Fu⁺, Yiguang Zhu⁺, Xiangui Mei, Yi Wang, Haijian Jia, Changsheng Zhang* and Weiming Zhu*. Acyclic Congeners from Actinoalloteichus cyangriseus Provide Insights into Cyclic Bipyridine Glycoside Formation. Organic Letters. 2014, 16(16): 4264-4267.

41. Yaolong Chen⁺, Wenjun Zhang⁺, Yiguang Zhu*, Qingbo Zhang, Xinpeng Tian, Si Zhang,and Changsheng Zhang*. Elucidating Hydroxylation and Methylation Steps Tailoring Piericidin A1 Biosynthesis. Organic Letters. 2014, 16(3): 736-739.

40. Wenjun Zhang, Sumei Li, Yiguang Zhu, Yuchan Chen, Yaolong Chen, Haibo Zhang, Guangtao Zhang, Xinpeng Tian, Yu Pan, Si Zhang, Weimin Zhang, and Changsheng Zhang*. Heronamides D–F, Polyketide Macrolactams from the Deep-Sea-Derived Streptomyces sp. SCSIO 03032. Journal of Natural Products. 2014, 77(2): 388-391.

39. Wenjun Zhang⁺, Liang Ma⁺, Sumei Li, Zhong Liu, Yuchan Chen , Haibo Zhang, Guangtao Zhang, Qingbo Zhang, Xinpeng Tian , Chengshan Yuan, Si Zhang , Weimin Zhang, and Changsheng Zhang*. Indimicins A–E, Bisindole Alkaloids from the Deep-Sea-Derived Streptomyces sp. SCSIO 03032. Journal of Natural Products. 2014, 77(8): 1887-1892.

2013

38. Ruidong Chen⁺, Haibo Zhang⁺, Gaiyun Zhang, Sumei Li, Guangtao Zhang, Yiguang Zhu, Jinsong Liu, Changsheng Zhang*. Characterizing Amosamine Biosynthesis in Amicetin Reveals AmiG as a Reversible Retaining Glycosyltransferase. Journal of the American Chemical Society. 2013, 135(33): 12152-12155.

37. Yiguang Zhu, Qingbo Zhang, Sumei Li, Qinheng Lin, Peng Fu, Guangtao Zhang, Haibo Zhang, Rong Shi, Weiming Zhu, Changsheng Zhang*. Insights into Caerulomycin A Biosynthesis:A Two-Component Monooxygenase CrmH-Catalyzed Oxime Formation. Journal of the American Chemical Society. 2013, 135(50): 18750-18753.

36. Sumei Li, Ji Xiao, Yiguang Zhu, Guangtao Zhang, Chunfang Yang, Haibo Zhang, Liang Ma, Changsheng Zhang*. Dissecting Glycosylation Steps in Lobophorin Biosynthesis Implies an Iterative Glycosyltransferase. Organic Letters. 2013, 15(6): 1374-1377.

35. Zhengchao Wu, Sumei Li, Jie Li, Yuchan Chen, Kumar Saurav, Qingbo Zhang, Haibo Zhang, Wenjun Zhang, Weimin Zhang, Si Zhang, Changsheng Zhang*. Antibacterial and Cytotoxic New Napyradiomycins from the Marine-Derived Streptomyces sp. SCSIO 10428. Marine Drugs. 2013, 11(6): 2113-2125.

34. Ji Xiao, Qingbo Zhang, Yiguang Zhu, Sumei Li, Guangtao Zhang, Haibo Zhang, Kumar Saurav, Changsheng Zhang*. Characterization of the sugar-O-methyltransferase LobS1 in lobophorin biosynthesis. Applied Microbiology and Biotechnology. 2013, 97(20): 9043-9053.

33. Zhengchao Wu, Sumei Li, Sangjip Nam, Zhong Liu, Changsheng Zhang*. Nocardiamides A and B, Two Cyclohexapeptides from the Marine-Derived Actinomycete Nocardiopsis sp. CNX037. Journal of Natural Products. 2013: 76: 694-701.

32. Qingbo Zhang, Sumei Li, Yuchan Chen, Xinpeng Tian, Haibo Zhang, Guangtao Zhang, Yiguang Zhu,Si Zhang, Weimin Zhang, Changsheng Zhang*. New diketopiperazine derivatives from a deep-sea-derived Nocardiopsis alba SCSIO 03039. Journal of Antibiotics. 2013, 66(1): 31-36.

2012

31. Yiguang Zhu, Peng Fu, Qinheng Lin, Guangtao Zhang, Haibo Zhang, Sumei Li, Jianhua Ju, Weiming Zhu, Changsheng Zhang*. Identification of caerulomycin A gene cluster implicates a tailoring amidohydrolase. Organic Letters. 2012, 14(11): 2666-2669.

30. Wenjun Zhang, Zhong Liu, Sumei Li, Tingting Yang, Qingbo Zhang, Liang Ma, Xinpeng Tian, Haibo Zhang, Caiguo Huang, Si Zhang, Jianhua Ju, Yuemao Shen , and Changsheng Zhang*. Spiroindimicins A-D: new bisindole alkaloids from a deep-sea-derived actinomycete.. Organic Letters. 2012, 14(13): 3364-3367.

29. Qingbo Zhang, Huixian Li, Sumei Li, Yiguang Zhu, Guangtao Zhang, Haibo Zhang, Wenjun Zhang, Rong Shi, and Changsheng Zhang*. Carboxyl Formation from Methyl via Triple Hydroxylations by XiaM in Xiamycin A Biosynthesis. Organic Letters. 2012, 14 (24): 6142–6145.

28. Huixian Li⁺, Qingbo Zhang⁺, Sumei Li, Yiguang Zhu, Guangtao Zhang, Haibo Zhang, Xinpeng Tian, Si Zhang, Jianhua Ju, Changsheng Zhang*. Identification and characterization of xiamycin A and oxiamycin gene cluster reveals an oxidative cyclization strategy tailoring indolosesquiterpene biosynthesis. Journal of the American Chemical Society. 2012, 134(21): 8996-9005.

27. Gaiyun Zhang, Haibo Zhang, Sumei Li, Ji Xiao, Yiguang Zhu, Siwen Niu, Jianhua Ju, Changsheng Zhang*. Characterization of the amicetin biosynthesis gene cluster from Streptomyces vinaceusdrappus NRRL 2363 implicates two alternative strategies for amide bond formation. Applied and Environmental Microbiology. 2012, 78(7): 2393-2401.

26. Qingbo Zhang, Attila Mandi, Sumei Li, Yuchan Chen, Wenjun Zhang, Xinpeng Tian, Haibo Zhang, Huixian Li, Weimin Zhang, Si Zhang, Jianhua Ju, Tibor Kurtan, Changsheng Zhang*. N-N-Coupled Indolo-sesquiterpene Atropo-Diastereomers from a Marine-Derived Actinomycete. European Journal of Organic Chemistry. 2012, 27, 5256-5262.

25. Wenjun Zhang, Zhong Liu, Sumei Li, Yongzhi Lu, Yuchan Chen,Haibo Zhang, Guangtao Zhang, Yiguang Zhu,Gaiyun Zhang, Weimin Zhang,Jinsong Liu, and Changsheng Zhang*. Fluostatins I-K from the South China Sea-Derived Micromonospora rosaria SCSIO N160. Journal of Natural Product. 2012, 75(11): 1937-1943.

2011

24. Yi Xiao⁺, Sumei Li⁺, Siwen Niu, Liang Ma, Guangtao Zhang, Haibo Zhang, Gaiyun Zhang, Jianhua Ju and Changsheng Zhang*. Characterization of Tiacumicin B Biosynthetic Gene Cluster Affording Diversified Tiacumicin Analogues and Revealing a Tailoring Dihalogenase, Journal of the American Chemical Society. 2011, 133(4): 1092-1105.

23. Siwen Niu⁺, Tao Hu⁺, Sumei Li, Yi Xiao, Liang Ma, Gaiyun Zhang. Haibo Zhang, Si Zhang, Xiaohong Yang and Changsheng Zhang*. Characterization of a Sugar-O-methyltransferase TiaS5 Affords New Tiacumicin Analogues with Improved Antibacterial Property and Reveals Substrate Promiscuity. ChemBioChem. 2011, 12(11): 1740-1748.

22. Sumei Li, Xinpeng Tian, Siwen Niu, Wenjun Zhang, Yuchan Chen, Haibo Zhang, Weimin Zhang, Wenjun Li, Si Zhang, Jianhua Ju and Changsheng Zhang*. Pseudonocardians A-C, New Diazaanthraquinone Derivatives from a Deep-Sea Actinomycete Pseudonocardia sp. SCSIO 01299. Marine Drugs. 2011, 9(8): 1428-1439.

21. Siwen Niu, Sumei Li, Yuchan Chen, Xinpeng Tian, Haibo Zhang, Guangtao Zhang, Weimin Zhang, Xiaohong Yang, Si Zhang, Jianhua Ju and Changsheng Zhang*. Lobophorins E and F, new spirotetronate antibiotics from a South China Sea-derived Streptomyces sp. SCSIO 01127. The Journal of Antibiotics. 2011, 64(11): 711-716.

20. Liping Gong, Yi Xiao, Qiang Liu, Sumei Li, Changsheng Zhang*, Jinsong Liu*. Preliminary X-ray crystallographic analysis of the glycosyltransferase from a marine Streptomyces species. Acta Crystallographica Section F-Structural Biology Communications. 2011, 67(1): 136-139.

19. Gavin J Williams, Jie Yang, Changsheng Zhang, Thorson Jon.S.*. Recombinant E. coli Prototype Strains for in Vivo Glycorandomization. ACS Chem. Biol., 2011, 6(1): 94-100.

2002-2010

18. Changsheng Zhang, Rocco Moretti, Jiqing Jiang, Thorson JS Jon S. Thorson*. The In Vitro Characterization of Polyene Glycosyltransferases AmphDI and NysDI. ChemBioChem. 2008, 9(15): 2506-2514.

17. Changsheng Zhang, Bitto Eduard, Goff Randal D, Singh, Shanteri, Bingman, Craig A, Griffith Byron R, Albermann Christoph, Phillips George N. Jr* and Jon S. Thorson*, Biochemical and Structural Insights of the Early Glycosylation Steps in Calicheamicin Biosynthesis. Chemistry & Biology. 2008, 15(8): 842-853.

16. Williams Gavin J., Goff Randal D., Changsheng Zhang, Jon S. Thorson*. Optimizing glycosyltransferase specificity via ‘hot spot’ saturation mutagenesis presents a new catalyst for novobiocin glycorandomization. Chemistry & Biology. 2008, 15(4): 393-401.

15. Singh Shanteri, McCoy, Jason G., Changsheng Zhang, Bingman Craig A., Phillips George N., Jr.*, Jon S. Thorson*. Structure and Mechanism of the Rebeccamycin Sugar 4-O-Methyltransferase RebM. Journal of Biological Chemistry. 2008, 283(33): 22628-22636.

14. Changsheng Zhang, Qiang Fu, Albermann Christoph, Lingjun Li, Jon S. Thorson*, The In Vitro Characterization of the Erythronolide Mycarosyltransferase EryBV and Its Utility in Macrolide Diversification. ChemBioChem. 2007, 8(4): 385-390.

13. Changsheng Zhang, Albermann Christoph, Xun Fu, Jon S. Thorson*, The In Vitro Characterization of the Iterative Avermectin Glycosyltransferase AveBI Reveals Reaction Reversibility and Sugar Nucleotide Flexibility. Journal of the American Chemical Society. 2006, 128(51): 16420-16421.

12. Changsheng Zhang, Griffith Byron R, Qiang Fu, Albermann Christoph, Xun Fu, Lee In Kyoung, Lingjun Li, Jon S. Thorson*. Exploiting the Reversibility of Natural Product Glycosyltransferase-catalyzed Reactions. Science. 2006, 313(5791): 1291-1294.

11. Changsheng Zhang, Albermann Christoph, Xun Fu, Peters Noel R, Chisholm John D, Guisheng Zhang, Gilbert Eric J, Wang Peng George, Van Vranken DavidL, Jon S. Thorson*. RebG- and RebM-catalyzed Indolocarbazole Diversification. ChemBioChem. 2006, 7(5): 795-804.

10. Changsheng Zhang, Rachel L Weller, Jon S. Thorson, Scott R. Rajski*. Natural Product Diversification Using Non-natural Cofactor Analogues of S-adenosyl-L-methionine. Journal of the American Chemical Society. 2006, 128(9): 2760-2761.

9. Svetlana A. Borisova, Changsheng Zhang, Haruko Takahashi, Hua Zhang, Alexander W. Wong, Jon S. Thorson, Hung-wen Liu *. Substrate Specificity of the Macrolide-Glycosylating Enzyme Pair DesVII/DesVIII: Opportunities, Limitations and Mechanistic Hypotheses. Angewandte Chemie International Edition. 2006, 45(17): 2748-2753.

8. Qunjie Gao, Changsheng Zhang, Blanchard Sophie, Jon S. Thorson *. Deciphering indolocarbazole and enediyne aminopentose biosynthesis through comparative genomics: New insights and tools from the Actinomadura melliaura AT2433 biosynthetic locus. Chemistry & Biology. 2006, 13(7): 733-743.

7. Singh Shanteri, Hager Martin H., Changsheng Zhang, Griffith Byron R., Lee Min S, Hallenga Klaas, Markley John L. and Jon S. Thorson*. Structural insight into self-sacrifice mechanism of enediyne resistance. ACS Chem. Biol., 2006, 1(7): 451-460.

6. Fu X, Albermann C, Zhang C, Thorson JS*. Diversifying Vancomycin via Chemoenzymatic Strategies. Organic Letters. 2005, 7(8): 1513-1515.

5. Changsheng Zhang, Michael Podeschwa, Oliver Block, Hans Jpsef Altenbach, Wolfgang Piepersberg and Udo F Wehmeier*. Identification of a 1-epi-valienol 7-kinase activity in the producer of acarbose, Actinoplanes sp. SE50/110. FEBS Letters. 2003, 540(1-3): 53-57.

4. Changsheng Zhang, Michael Podeschwa, Hans Jpsef Altenbach, Wolfgang Piepersberg and Udo F Wehmeier*. The acarbose-biosynthetic enzyme AcbO from Actinoplanes sp. SE 50/110 is a 2-epi-5-epi-valiolone-7-phosphate 2-epimerase. FEBS Letters, 2003, 540(1-3): 47-52.

3. Xun Fu, Christoph Albermann, Jiqing Jiang, Jianchun Liao, Changsheng Zhang & Jon S. Thorson *. Antibiotic optimization via in vitro glycorandomization. Nature Biotechnology. 2003, 21(12): 1467-1469.

2. Changsheng Zhang, Ansgar Stratmann, Oliver Block, Brückner Ralph, Michael Podeschwa, Hans Jpsef Altenbach, Udo F. Wehmeier and Wolfgang Piepersberg*. Biosynthesis of the C7-cyclitol Moiety of Acarbose in Actinoplanes Species SE50/110. 7-O-phosphorylation of the initial cyclitol precursor leads to proposal of a new biosynthetic pathway. Journal of Biological Chemistry.2002, 277(25): 22853-22862.

1. Changsheng Zhang, Ansgar Stratmann, Oliver Block, Brückner Ralph, Michael Podeschwa, Hans Jpsef Altenbach, Udo F. Wehmeier and Wolfgang Piepersberg*. Biosynthesis of the C7-cyclitol Moiety of Acarbose in Actinoplanes Species SE50/110. 7-O-phosphorylation of the initial cyclitol precursor leads to proposal of a new biosynthetic pathway. The Journal of Biological Chemistry. 2002, 277(25): 22853-22862.

授权专利

1. 张长生，李慧贤，张庆波，李苏梅，朱义广，张光涛，张海波，田新朋，张偲，鞠建华，厦霉素A和氧厦霉素的生物合成基因簇及其应用，ZL201210146664.x；

2. 张长生，张海波，李苏梅，张改云，张光涛，朱义广，陈瑞东，肖吉，鞠建华，友菌素的生物合成基因簇及其应用，ZL201210030835.2；

3. 张长生，张庆波，李苏梅，田新朋，张文军，张海波，李惠贤，张偲，鞠建华，链霉菌和吲哚倍半萜类化合物及其制备方法和在制备抗菌抗肿瘤药物中的应用，ZL201210146953.x；

4. 张长生，张文军，李苏梅，张海波，张改云，张光涛，朱义广，一种小单孢菌及利用该菌制备多种抗生素的方法，ZL201210467946.X；

5. 张长生，李苏梅，田新朋，牛四文，张文军，鞠建华，张偲一种链霉菌及利用该菌发酵制备抗霉素类抗生素的工艺，ZL201010520348.5；

6. 张长生，李苏梅，田新朋，牛四文，鞠建华，张偲，一种链霉菌及利用该菌制备多种抗生素的工艺，ZL201010603179.1；

7. 张长生，李苏梅，肖毅，牛四文，鞠建华，四种台勾霉素类化合物及其制备方法和在制备抗菌药物中的应用，ZL201010526416.9；

8. 张长生，李苏梅，田新朋，张文军，张海波，张光涛，张偲，鞠建华，抗生素Pseudonocardian A和B及其制备方法和在制备抗菌、抗肿瘤药物中的应用，ZL201110231993.X；

9. 张长生，李苏梅，牛四文，田新朋，张光涛，张海波，张偲，鞠建华，抗生素Lobophorin E和F及其制备方法和在制备抗菌、抗肿瘤药物中的应用，ZL201110149558.2；

10. 张长生，牛四文，胡涛，李苏梅，肖毅，马亮，张光涛，张海波，鞠建华，六种台勾霉素类化合物及其制备方法和在制备抗菌药物中的应用，ZL201110104051.5；

11. 张长生，张文军，田新朋，李苏梅，张庆波，马亮，张海波，张偲，鞠建华，链霉菌、抗肿瘤化合物Spiroindimycin A-D及其制备方法和应用，ZL201210087537.7；

12. 张长生，肖毅，李苏梅，牛四文，张光涛，张海波，胡涛，鞠建华，台勾霉素的生物合成基因簇及其应用，ZL201010592416.9；

13. 张长生，张文军，田新朋，李苏梅，张庆波，马亮，张海波，张偲，鞠建华，链霉菌、抗肿瘤化合物Spiroindimycin A-D及其制备方法和应用，特许第5826406（PCT国际专利（日本）；

14. 张长生，吴正超，李苏梅，李洁，库马·萨乌拉夫，张庆波，张海波，张文军，张偲，链霉菌、新化合物及其制备方法和在制备抗菌抗肿瘤药物中的应用，ZL201310180810.5；

15. 张长生，张光涛，张文军，张庆波，朱义广，张海波，李苏梅，马亮，一种斑鸠霉素的生物合成基因簇及其应用，ZL201410120626.6；

16. 张长生，张文军，马亮，李苏梅，张庆波，张海波，张光涛，抗生素Indimicins A-E及其制备方法和在制备抗肿瘤药物中的应用，ZL201410214540.X；

17. 张长生，张文军，杨春芳，黄春帅，朱义广，抗生素difluostatin A及其制备方法和在制备抗菌药物中的应用，ZL201510650332.9；

18. 张长生，朱义广，张文军，陈耀龙，袁呈山，张海波，张光涛，张庆波，一种大环内酰胺类化合物heronamides的生物合成基因簇及其应用，中国，ZL201510404149.0；

19. 张长生，吴正超，李苏梅，李洁，库马·萨乌拉夫，张庆波，张海波，张文军，张偲，新化合物及其制备方法和在制备抗菌抗肿瘤药物中的应用，ZL201510512966.8；

20. 张长生，张光涛，张文军，张庆波，朱义广，张海波，李苏梅，马亮，一种斑鸠霉素的生物合成基因簇及其应用，ZL201510648554.7；

21. 张长生，张光涛，张文军，朱义广，袁呈山，张庆波，张海波，张丽萍，一种帕克特酰胺的生物合成基因簇及其应用，ZL 201610807614X；

22. 张长生，张文军，杨春芳，黄春帅，张丽萍，张海波，抗生素pyrazolofluostatin A-C及其制备方法和应用，ZL 201710008234.4；

23. 张长生，黄春帅，杨春芳，张文军，张丽萍，朱义广，蒋晓东，方春艳，张庆波，袁呈山，一种二聚fluostatins类抗生素的制备方法，ZL2018103338713

24. 张长生，蒋晓东，张庆波，朱义广，吴正超，聂方玲，杨春芳，一种非典型角环素类化合物nenestatin A的生物合成基因簇及其应用，ZL201710142626X

主持或参加科研项目（课题）情况

国家自然科学基金委项目：

——杰出青年基金 2012-2015

——重点项目 2017-2021

——重点国际（地区）合作研究项目（2）2019-2020；2019-2023

——面上项目（3）2009-2011；2011-2013；2015-2018

省部级项目：

——国家重点基础研究发展计划（973计划）课题  2010-2014

——国家海洋经济创新发展区域示范项目课题 2013-2016

中国科学院项目：

——中科院前沿领域重点项目 2016-2021

——重要方向性项目 2009-2011

——先导专项重点任务 2013-2017

广东省项目：

——海洋经济创新发展区域示范项目，课题 2013-2017

——自然基金重大培育项目 2015-2018

——特支计划人才项目（2） 2015-2019

——渔港建设和渔业产业发展专项 2016-2018

——促进经济发展专项（海洋经济发展用途）2018-2020

——海洋渔业科技与产业发展专项 2016-2018

海南省项目：

——重大科技计划项目 2020-2022

其他项目：

——南方海洋科学与工程广东省实验室（广州）人才团队引进重大专项 2019-2022

学科组建设

责任研究员：张长生

研究员：闫岩、朱义广（项目）、张文军（项目）

副研究员：张海波、张文军、张光涛、张庆波、杨春芳（项目）、张丽萍（项目）

特别研究助理：马亮

博士后：王利娟、刘威、朱梦奕、谭彬

行政人员：于艳玲

博士研究生：金红波（2015直博生）、BIDHAN CHANDRA DE（2016）、蒋晓东（2017）、IMRAN KHAN（2017）、陈思强（2018直博生）、方壮杰（2019）、方春艳（2020）

硕士研究生：翟诗岚（2018）、赵梦冉（2019）、张鑫雅（2019）、蒋鹏（2020）

已培养博士研究生8名；硕士研究生11名