龙丽娟研究员

中国科学院南海海洋研究所 副所长（主持工作）

（藻类资源与生态工程学科组责任研究员）

研究兴趣与领域

微型海洋生物资源(微生物和微藻）的发掘：采集、分类、鉴定和保藏。

代表性工作简介

长期从事南海海洋生物资源挖掘、功能利用与生态工程应用研究工作。围绕热带海洋生物多样性认知、功能挖掘和可持续利用，发展基于生态系统结构与功能完整性的生态保护、修复理论技术体系与应用示范，促进热带海洋生态系统保护和生物资源绿色利用的工程化。在海洋微型生物（微藻和微生物）种质资源库构建、菌种功能发掘与利用，海洋生物功能物质利用新技术，珊瑚礁藻类资源与生态工程应用方面取得一系列重要创新成果。

学习经历

1982.09-1986.07：中国海洋大学（山东海洋学院）海洋生物系海洋植物专业，学士学位

1986.09-1989.07：中国海洋大学（青岛海洋大学）水产学院微藻培养与应用专业，硕士学位

工作经历

1989-至今：中国科学院南海海洋研究所，副所长，责任研究员

2008.12-2009.03：美国马里兰大学，高级访问学者

获奖与荣誉

1. 海南科技进步奖特等奖（2004，第四）

2. 第十六届全国发明展览会金奖（2006，第五）

3. 国家科技进步二等奖（2007，第四）

4. 海南省科技进步一等奖（2010）

5. 广东省科技发明一等奖（2011，第三）

6. 广州优秀女科技工作者（2012）

7. 国家技术发明奖二等奖（2014，第二）

8. 国务院政府特殊津贴专家（2014）

9. 中科院特聘核心骨干研究员（2016）

10.广东省丁颖科技奖（2017）

11. 中国科学院杰出科技成就奖（1/3突出贡献者，2019）

12. 广东省科学技术（科技进步）一等奖（排名第一，2019）

学术兼职

1. 广州市海珠区科协副主席（2015-2020）

2. 中国海洋学会热带海洋分会秘书长（2012-2022）

3. 广东海洋学会理事（2012-至今）

4. 广东海洋湖沼学会（2012-至今）

5. 湛江市第三届科技兴湛高级顾问团高级顾问（2015-2020）

6.《热带海洋学报》副主编（2014-至今）

代表性学术成果

论文发表

25. Ru Li？, Jian Yang？, Yunzhu Xiao, Lijuan Long*. In vivo immobilization of an organophosphorus hydrolyzing enzyme on bacterial polyhydroxyalkanoate nano-granules. Microbial Cell Factories. 2019, 18: 166

24. Xinyuanyuan Sun, Sijun Huang, Lijuan Long*. Characterization, complete genome and proteome of a bacteriophage infecting a coral-derived Vibrio strain. Marine Genomics. 2019, 47, 1874-7787

23. Fangfang Yang, Wenzhou Xiang, Tao Li, Lijuan Long*. Transcriptome analysis for phosphorus starvation-induced lipid accumulation in Scenedesmus sp. Scientific reports. 2018,8:16420

22. Rou-Wen Chen+, Ke-Xin Wang, Fa-Zuo Wang, Yuan-Qiu He, Li-Juan Long*, Xin-Peng Tian*. Rubrobacter indicoceani sp. nov., a new marine actinobacterium isolated from Indian Ocean sediment. International Journal of Systematic and Evolutionary Microbiology. 2018, 68:3487-3493

21. Rou-Wen Chen, Ke-Xin Wang, Xue-Feng Zhou, Chao Long, Xin-Peng Tian*, Li-Juan Long*. Indioceanicola profundi gen. nov., sp. nov., isolated from Indian Ocean sediment. International Journal of Systematic and Evolutionary Microbiology. 2018,68:3707-3712

20. Yunzhu Xiao(#), Jian Yang(#), Xinpeng Tian, Xiaoxue Wang, Jie Li, Si Zhang, Lijuan Long(*). Biochemical basis for hydrolysis of organophosphorus by a marine bacterial prolidase, Process Biochemistry, 2017.01.01, 52: 141~148

19. Li, J., Kuang, W., Long, L., & Zhang, S. (2017). Production of quorum-sensing signals by bacteria in the coral mucus layer. Coral Reefs.

18. Xinpeng Tian, Zhewen Zhang, Tingting Yang, et al., Lijuan Long* and Jingfa Xiao*. Comparative Genomics Analysis of Streptomyces Species Reveals Their Adaptation to the Marine Environment and Their Diversity at the Genomic Level. Frontiers in Microbiology, 2016, 7:998. 27 June 2016

17. Li, Baolian, Zhang, Si, Long, Lijuan*, Huang, Sijun*. Characterization and Complete Genome Sequences of Three N4-Like Roseobacter Phages Isolated from the South China Sea   Current Microbiology, 2016, 73 (3), pp 409–418

16. F Yang, W Xiang, J Fan, H Wu, T Li, L Long. High pH-induced flocculation of marine Chlorella sp. for biofuel production- Journal of applied phycology, 2016 – Springer

15. Lizhen Li(#), Jian Yang(#), Jie Li, Lijuan Long, Yunzhu Xiao, Xinpeng Tian, Fazuo Wang, Si Zhang(*).Role of two amino acid residues' insertion on thermal stability of thermophilic alpha-amylase AMY121 from a deep sea bacterium Bacillus sp SCSIO 15121, Bioprocess and Biosystems Engineering, 2015, 38(5): 871~879

14. Jian Yang, Jie Li, Yunfeng Hu, Lizhen Li, Lijuan Long, Fazuo Wang, Si Zhang(*). Characterization of a thermophilic hemoglobin-degrading protease from Streptomyces rutgersensis SCSIO 11720 and its application in antibacterial peptides production, Biotechnology and Bioprocess Engineering, 2015, 20(1): 79~90

13. F Yang, C Cheng, L Long, Q Hu, Q Jia, H Wu. Extracting lipids from several species of wet microalgae using ethanol at room temperature. Energy & , 2015 - ACS Publications

12. Kuang, W., Li, J., Zhang, S., & Long, L. (2015). Diversity and distribution ofactinobacteriaassociated with reef coralporites lutea. Frontiers in Microbiology, 6, 1094.

11. Fang-Fang Yang,Wen-Zhou Xiang, Xiu-Mei Sun, Hua-Lian Wu, Tao Li, Li-Juan Long*. A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp. at Room Temperature. marine drugs. 2014,12,1258-1270

10. Jie Li, Qi Chen, Li-Juan Long, Jun-De Dong, Jian Yang, Si Zhang*, Bacterial dynamics within the mucus, tissue and skeleton of the coral Porites lutea during different seasons, Scientific Reports, 2014,4:7320

9. Lizhen Li, Jian Yang, Juan Li, Lijuan Long, Yunzhu Xiao, Xinpeng Tian, Fazuo Wang, Si Zhang*, Role of two amino acid residues’ insertion on thermal stability of thermophilic α-amylase AMY121 from a deep sea bacterium Bacillus sp. SCSIO 15121, Bioprocess and Biosystems Engineering, 2014,1-9

8. Jie Li, Qi Chen, Si Zhang, Hui Huang, Jian Yang, Xinpeng Tian, Lijuan Long*. Highly heterogeneous bacterial communities associated with the South China Sea reef corals Porites lutea, Galaxea fascicularis and Acropora millepora. PLoS ONE 2013,8(8):e71301

7. Tian, Xin-Peng, Long, Li-Juan, Wang, Fa-Zuo, Xu, Ying, Li, Jie, Zhang, Jing, Zhang, Chang-Sheng, Zhang, Si, Li, Wen-Jun，Streptomyces nanhaiensis sp nov., a marine streptomycete isolated from a deep-sea sediment. International Journal of Systematic and Evolutionary Microbiology, 2012,62(4)：864-868. 

6. Si Zhang, Lijuan Long, Effects of glycopeptides on development, growth and non-specific immunity of pearl oyster Pinctada fucata (Gould), Aquaculture Nutrition, 2010， 16（5）: 520-527.

5. Liang, Ji-Lin, Long, Li-Juan, Zhang, Si, Wu, Jun,Cooliatin, an Unprecedented Natural Dioxocyclononane from Dinoflagellate Coolia monotis from South China Sea. Chemical & Pharmaceutical Bulletin, 2009,57(8):888-889. 

4. Long L. J.,Song Y., Wu J., Lei L., Huang K., Long B. W., Development of an efficient method for the preparative isolation and purification of chlorophyll a from a marine dinoflagellate Amphidinium carterae by high-speed counter-current chromatography coupled with reversed-phase high-performance liquid chromatography. Analytical and Bioanalytical Chemistry. 2006, 386 (7-8): 2169-2174.

3. Yin, Hao, Zhang, Si, Wu, Jun, Nan, Haihan, Long, Lijuan, Yang, Jin, Li, Qingxin.Pongaflavanol: a prenylated flavonoid from Pongamia pinnata with a modified ring A Molecules, 2006,11(10), pp 786-791.

2. Wu, J, Long, LJ, Song, Y, Zhang, S, Li, QX, Huang, HS, Xiao, ZH,A new unsaturated glycoglycerolipid from a cultured marine dinofiagellate Amphidinium carterae. Chemical & Pharmaceutical Bulletin, 53(3):330-332, 2005.

1. Si Zhang*, Long Lijuan, Alkaloids from Marine Organisms, Modern Marine Medicine (Chief editor: Binghua Jiao)，2005，Beijing: Science Press, 260-293.

撰写专著

1.《西南沙岛礁关键造护礁功能生物》，龙丽娟、殷建平、林强等，科学出版社，2019

授权专利

1. 海洋链霉菌、吡喃型倍半萜类化合物及其制备方法和用途，ZL201110460246.3，龙丽娟，田新朋，李洁，罗雄明，齐振雄，尹团

2. 一种微生物包埋微球连续制备装置及方法，ZL 201410469754.1，龙丽娟、李洁、吴家法、杨键、张偲.

3. 一类热稳定性提高的淀粉酶突变体及其编码基因和应用，ZL201510446883，龙丽娟 杨键 李丽珍 李洁 肖运柱 齐振雄 尹团 张偲

4. 一个海洋来源小单孢菌 SCSIO 01819及其在珍珠加工和功能水产饲料蛋白源中的应用  ZL201510366099.1，龙丽娟、尹浩、田新朋、齐振雄、尹团、张偲

5. 一类热稳定性提高的淀粉酶突变体及其编码基因和应用，ZL201510446883.3，龙丽娟，杨键，李丽珍，李洁，肖运柱，齐振雄，尹团，张偲. .

6. 一种二肽酶突变体及其编码基因和应用，ZL201610705142.7，龙丽娟，杨键，肖运柱，田新朋，张偲

学科组建设

责任研究员：龙丽娟

研究员：田新朋

副研究员：黄思军、杨键、杨芳芳（项目）

特别研究助理：韦章良

行政人员：潘少玲

博士研究生：龙超（2016）、李茹（2017）、张建东（2018）、刘玉（2019）

硕士研究生：莫嘉豪（2017）、李福润（2018）、肖志梁（2019）

已培养博士研究生4名；硕士研究生9名