黄晓红研究员
副标题:
职务描述
1. 中国科学院南海海洋研究所 硕士生导师
研究方向
主要研究鱼类病毒感染的分子机制及免疫防控。近年来,从事水生动物病毒致病机理、病原防控、鱼类免疫学及海洋动物细胞培养研究工作,在鱼类两栖和爬行动物虹彩病毒感染致病的分子机理研究、功能基因鉴定、病毒与宿主细胞相互作用及鱼类细胞培养等方面取得一系列研究成果。
教育背景和工作经历
2001.7 河南师范大学生命科学学院获得农学学士学位
2006.7 中国科学院水生生物研究所水生生物学博士学位
2006.10-2009.2 中山大学博士后研究工作
2009.3-至今 中科院南海海洋研究所
承担项目
1. 国家基金面上项目“利用荧光标记重组病毒研究石斑鱼虹彩病毒感染动力学和抗病毒药物筛选【31172445】”
2012.1.1-2015.12.31
2. 国家基金面上项目” 石斑鱼泛素蛋白酶体系统对虹彩病毒SGIV感染复制的调节作用机制【31372566】”
2014.1.1-2017.12.31
3. 国家863课题” 海水养殖鱼类主要病毒和细菌性病原亚单位疫苗的研发【2014AA093507】” 2014.1.1-2016.12.31
代表性论文
2014:
1.Wang SW#, Huang XH#, Huang YH, Hao X, Xu HJ, Cai MJ, Wang HD, Qin QW*. A novel marine DNA virus (Singapore grouper iridovirus, SGIV) entry into host cells occurs via clathrin-mediated endocytosis and macropinocytosis in a pH-dependent manner. Journal of Virology. 2014,88:13047-13063
2.Ren Gao, Youhua Huang, Xiaohong Huang, Guan L, Shina Wei, Zhou Y, Qiwei Qin*. Molecular cloning and characterization of two types of IκBα orthologues in orange-spotted grouper, Epinephelus coioides. Fish & Shellfish Immunology. 2014,38(1):101-110
3.Jing Fu, Youhua Huang, Jia Cai, Shina Wei, Zhengliang Ouyang, Feng Ye, Xiaohong Huang, Qiwei Qin*. Identification and characterization of Rab7 from orange-spotted grouper, Epinephelus coioides. Fish & Shellfish Immunology. 2014,36(1):19-26.
4.Shina Wei, Youhua Huang, Xiaohong Huang, Jia Cai, Yang Yan, Chuanyu Guo, Qiwei Qin*. Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection. Fish & Shellfish Immunology. 2014,36(1):194-205
5.Huang XH#, Wang W, Huang YH, Xu LW, Qin QW*. Involvement of the PI3K and ERK signaling pathways in largemouth bass virus-induced apoptosis and viral replication. Fish & Shellfish Immunology. 2014,41:371-379
6.Huang XH#, Huang YH, Yang Y, Wei SN, Qin QW*. Involvement of fish signal transducer and activator of transcription 3 (STAT3) in SGIV replication and virus induced paraptosis. Fish & Shellfish Immunology. 2014,41:308-316
7.Gao R, Huang YH, Huang XH, Guan LY, Wei SN, Zhou YS*, Qin QW*. Molecular cloning and characterization of two types of IkBa orthologues in orange-spotted grouper, Epinephelus coioides. Fish & Shellfish Immunology. 2014,38: 101-110
8.Fu J, Huang YH, Cai J, Wei SN, Ouyang ZL, Ye FZ, Huang XH*, Qin QW*. Identification and charactirization of Rab7 from orange-spotted grouper , Epinephelus Coioides. Fish & Shellfish Immunology. 2014,36:19-26
9.Huasong Ji, Jingguang Wei, Shina Wei, Yang Yan, Youhua Huang, Xiaohong Huang, Sheng Zhou, Zhou Y, Qiwei Qin*. Molecular cloning and expression of a C-type lectin-like protein from orange-spotted grouper Epinephelus coioides. Journal of Fish Biology. 2014,84(2):436-47
10.Huang YH, Huang XH, Ouyang ZL, Wei SN, Guo CY, Qin QW*. Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis. Aquaculture. 2014,432:265-272
2013:
1.Jia Cai, Youhua Huang, Shina Wei, Zhengliang Ouyang, Xiaohong Huang*, Qiwei Qin. Characterization of LPS-induced TNFα factor (LITAF) from orange-spotted grouper, Epinephelus coioides. Fish & Shellfish Immunology. 2013,35:1858-1866
2.Xiaohong Huang, Youhua Huang, Jia Cai, Shina Wei, Zhengliang Ouyang, Qiwei Qin*. Molecular cloning, expression and functional analysis of ISG15 in orange-spotted grouper, Epinephelus coioides. Fish & Shellfish Immunology. 2013,34(5):1094-1102
3.Chuanyu Guo,Yang Yan, Huachun Cui, Xiaohong Huang, Qiwei Qin*. miR-homoHSV of Singapore Grouper Iridovirus (SGIV) Inhibits Expression of the SGIV Pro-apoptotic Factor LITAF and Attenuates Cell Death. PLoS ONE. 2013,8:e83027
4.Yang Yan, Huachun Cui, Chuanyu Guo, Li J, Xiaohong Huang, Jingguang Wei, Qiwei Qin*. An insulin-like growth factor homologue of Singapore grouper iridovirus modulates cell proliferation, apoptosis and enhances viral replication. Journal of General Virology. 2013,94:2759-2770
5.Xiaohong Huang, Youhua Huang, Jia Cai, Shina Wei, Ren Gao, Qiwei Qin*. Identification and characterization of a tumor necrosis factor receptor like protein encoded by Singapore grouper iridovirus. Virus Research. 2013,178:340-348
2012:
1.Wei JG, Guo ML, Ji HS, Yan Y, Ouyang ZL, Huang XH, Hang YH, Qin QW*. Grouper translationally controlled tumor protein prevents cell death and inhibits the replication of Singapore grouper iridovirus (SGIV). Fish and Shellfish Immunology. 2012, 33:916-925
2.Guo ML, Wei JG, Huang XH, Huang YH, Qin QW*. Antiviral effects of β-defensin derived from orange-spotted grouper (Epinephelus coioides). Fish and Shellfish Immunology. 2012, 32: 828-838.
3.Wei SN, Huang YH, Cai J, Huang XH, Fu J, Qin QW*. Molecular cloning and characterization of c-type lysozyme gene in orange-spotted grouper, Epinephelus coioides. Fish & Shellfish Immunology. 2012, 33: 186-196
4.Ouyang ZL, Wang PR, Huang YH, Huang HX, Wan QJ, Zhou S, Wei JG, Zhou YC, Qin QW*. Selection and identification of Singapore grouper iridovirus vaccine candidate antigens using bioinformatics and DNA vaccination. Veterinary Immunology and Immunopatholog. 2012,149:38-45
5.Wei JG, Guo ML, Ji HS, Yan Y, Ouyang ZL, Huang XH, Huang YH, Qin QW*. Cloning, characterization, and expression analysis of a thioredoxin from orange-spotted grouper (Epinephelus coioides). Developmental and Comparative Immunology. 2012.38: 108-116
6.ZL Ou-yang, PR Wang, XH Huang, J Cai, YH Huang, SN Wei, HS Ji JG Wei, YC Zhou, QW Qin*. Immunogenicity and protective effects of inactivated Singapore grouper iridovirus (SGIV) vaccines in orange-spotted grouper, Epinephelus coioides. Developmental and Comparative Immunology. 2012, 38:254-261
7.Yang Yan, HuaChun Cui, JingGuang Wei, YouHua Huang, XiaoHong Huang, QiWei Qin*. Functional genomic studies on an immune- and antiviral-related gene of MyD88 in orange-spotted grouper, Epinephelus coioides. Chinese Science Bulletin. 2012, 57: 3277-3287
2011:
1.Cui HC, Yan Y, Wei JG, Huang XH, Huang YH, Ouyang ZL, Qin QW*. Identification and functional characterization of an interferon regulatory factor 7-like (IRF7-like) gene from orange-spotted grouper, Epinephelus coioides. Developmental and Comparative Immunology. 2011, 35: 672–684.
2.Huang, YH., Huang, X H., Yan, Y., Cai, J., Ouyang, ZL.,Cui, HC, Wang, PR., Qin, Q W*. Transcriptome analysis of orange-spotted grouper (Epinephelus coioides) spleen in response to Singapore grouper iridovirus. BMC Genomics. 2011. 12:556.
3.Cai, J., Huang, YH., Wei, SN., Huang, XH., Ye, FZ., Fu, J., Qin, QW*..Characterization of p38 MAPKs from orange-spotted grouper, Epinephelus coioides involved in SGIV infection. Fish and Shellfish Immunology. 2011 31: 1129-1136.
4.Huang, XH., Huang, YH., Ouyang, ZL., Xu, LX., Yan, Y., Cai, J., Cui, HC., Han, X., Qin, QW*. Singapore grouper iridovirus, a large DNA virus, induces nonapoptotic cell death by a cell type dependent fashion and evokes ERK signaling. Apoptosis. 2011.16:831–845.
5.Yan Y, Cui HC, Jiang S, Huang YH, Huang XH, Wei SN, Xu W, Qin QW*. Identification of a Novel Marine Fish Virus, Singapore Grouper Iridovirus-Encoded MicroRNAs Expressed in Grouper Cells by Solexa Sequencing. PloS ONE. 2011. 6(4): e19148.
6.Zhou S, Wan QJ, Huang YH, Huang XH, Cao JH, Ye LL, Lim TK, Lin QS, Qin QW*. Proteomic analysis of Singapore grouper iridovirus envelope proteins and characterization of a novel envelope protein VP088. Proteomics. 2011, 11, 2236–2248.
7.Zhou JG, Wei JG, Xu D, Cui HC, Yan Y, Ou-Yang ZL, Huang YH, Huang XH, Qin QW*. Molecular cloning and characterization of two novel hepcidins from orange-spotted grouper, Epinephelus coioides. Fish and Shellfish Immunology. 2011, 30: 559-568.
8.Huang YH, Huang XH, Cai J, Ye FZ, Qin QW*. Involvement of the mitogen-activated protein kinase pathway in soft-shelled turtle iridovirus-induced apoptosis. Apoptosis. 2011, DOI 10.1007/s10495-011-0595-z
2010:
1.Wei JG, Xu D, Zhou JG, Cui HC, Yan Y, Ouyan ZL, Gong J, Huang YH, Huang XH, Qin QW*. Molecular cloning, characterization and expression analysis of a C-type lectin (Ec-CTL) in orange-spotted grouper, Epinephelus coioides. Fish and Shellfish Immunology. 2010,28(1): 178-186
2009:
1.Huang X, Huang Y, Sun JJ, Han X, Qin QW*. Characterization of two grouper Epinephelus akaara cell lines: Application to studies of Singapore grouper iridovirus (SGIV) propagation and virus–host interaction. Aquaculture. 2009, 292: 172-179.
2.Huang Y, Huang X, Liu H, Gong J, Ouyang Z, Cui H, Cao J, Zhao Y, Wang X, Jiang Y, Qin QW*. Complete sequence determination of a novel reptile iridovirus isolated from soft-shelled turtle and evolutionary analysis of Iridoviridae. BMC Genomics. 2009 May 14;10:224.
授权专利:
1.一种石斑鱼虹彩病毒囊膜蛋白的提取方法,ZL201110110422.0,秦启伟,欧阳征亮,公杰,黄晓红,黄友华
2.一种石斑鱼虹彩病毒灭活疫苗的制备方法,ZL201110110399.5,秦启伟,欧阳征亮,汪沛然,黄晓红,黄友华
研究生培养
硕士研究生:王伟,杨莹